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Rapid, microplate-based fluorogenic screening of a positional scanning combinatorial library accurately predicts the sequence specificity of metal-assisted peptide hydrolysis. 相似文献
93.
Ryan E Campbell-Anson Diane Kentor Yi J Wang Kathryn M Bushnell Yufeng Li Luis M Vence Laszlo G Radvanyi 《Journal of immune based therapies and vaccines》2008,6(1):1
Background
Human monocyte-derived DC (mDC) loaded with peptides, protein, tumor cell lysates, or tumor cell RNA, are being tested as vaccines against multiple human malignancies and viral infection with great promise. One of the factors that has limited more widespread use of these vaccines is the need to generate mDC in large scale. Current methods for the large-scale cultivation of mDC in static culture vessels are labor- and time- intensive, and also require many culture vessels. Here, we describe a new method for the large-scale generation of human mDC from human PBMC from leukopheresis or buffy coat products using roller bottles, never attempted before for mDC generation. We have tested this technology using 850 cm2 roller bottles compared to conventional T-175 flat-bottom static culture flasks. 相似文献94.
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The differences between a full Lorentz-Lorenz potential modification, and a ?2 approximation to this modification are examined for low energy pion-nucleus elastic scattering. The relationship between these approaches and the single-scattering impulse approximation is also investigated. 相似文献
99.
Consider a continuous local martingale X. We say that X satisfies the representation property if any martingale Y of X can be represented as stochastic ITǒ integral of X. Using the method of random time change systematically, in the present paper the representation problem for continuous local martingales is treated. We describe a class of martingales Y that can be represented as stochastic integral of X by probabilistic conditions. This leads to sufficient conditions for the representation property of X being true. Besides, an interesting characterization of continuous processes with independent increments is obtained. In part II. we proceed with general examples, applications to the n-dimensional case, and, in particular, to the n-dimensional time change of continuous local martingales with orthogonal components. 相似文献
100.
The present study reports on experimental investigations about the phase velocity diagrams of Alfvén waves in the anisotropic solid-state plasma of bismuth. Alfvén-wave transmission at 34 GHz and 4.2 K has been observed, using an interference technique of high sensitivity. The experimental results are in good agreement with a theoretical model given by Buchsbaum, which makes only use of the anisotropic mass tensor of bismuth. 相似文献